MEGA SAMPLES VOL-78 [PC MAC]
MEGA SAMPLES VOL-78 [PC MAC] ->->->-> https://urloso.com/2tDYQP
All the Samples From Mars is the culmination of 9 years (and tens of thousands of hours) of sampling our favorite, most inspiring (often rare and vintage) drum machines, synthesizers, custom vinyl, and more. Recorded through some of the best recording equipment in the world, all of our samples feature 100% hardware processing - no plugins. And every sample has been painstakingly mapped for various types of software and hardware. We craft everything by hand in our studio:
We capture the cleanest possible samples (we've spent years sampling and at this point may have more experience than anybody else in the world doing so). This starts with making sure the drum machine is serviced, plugging it into the cleanest possible power, high end DIs and our API 1608 console. We record through high quality conversion (Apogee Symphony MKII) and often spice things up with a mastering reel to reel, and a host of mastering level hardware saturators, EQs, and compressors we've collected over the years. Most of our drum products include clean and color samples. The following are demos composed of the one shot drum samples:
Clade 7 resolves sections Restricti and Aspergillus in one clade (Fig. 8). The aligned data set was 610 bp long and the K2 + G + I model was used for ML analysis. Within the clade, we isolated one new species closely related to A. glaucus and A. proliferans, the latter also found in the dust samples together with A. chevalieri, A. montevidensis, A. penicillioides, A. pseudoglaucus, A. restrictus and A. ruber.
Clade 1 contains section Citrina (Fig. 13), a group of species of wide distribution and isolated from a wide range of sources (Houbraken et al. 2011b). The aligned data set was 448 bp long, with the K2 + G model selected for ML analysis. Species were identified as P. citrinum, P. pancosmium, P. roseopurpureum, P. sanguifluum, P. sizovae, P. steckii and P. sumatraense. Penicillium pancosmium was abundant in samples collected from South Africa, Indonesia and Micronesia. It is also extremely common in isolations from house dust samples collected in Regina, Canada (Hirooka, Tanney & Seifert, unpubl.).
Clade 11 comprises the recently reviewed section Chrysogena (Houbraken et al. 2012) (Fig. 23). This group of species was well represented in dust samples, especially P. rubens and to a lesser degree P. chrysogenum. The aligned data set was 444 bp long and the K2 + G model selected for the ML analysis. Isolates were identified as P. allii-sativii, P. chrysogenum, P. halotolerans, P. lanosocoeruleum and P. rubens.
The origin of common indoor species is difficult to determine. Aspergillus sydowii is a good example. We found A. sydowii to be one of the most common species in collected dust samples and the species is generally considered as widespread. The species is often isolated from soil (Domsch et al. 1980), is very common on mouldy gypsum wallboard, dust, paint and various foods (Gorbushina et al. 2007, Samson et al. 2010, Flannigan et al. 2011) and is commonly found in marine environments where it acts as an opportunistic pathogen of sea corals (Roth et al. 1964, Smith et al. 1996, Geiser et al. 1998, Toledo-Hernández et al. 2008, Rypien et al. 2008, Rypien 2008, Kirkwood et al. 2009). The source or origin of this species is still unknown, even though most studies suggest it being a terrestrial soil-borne fungus. The suggestion thus is that A. sydowii, along with a number of other soil-borne fungi, gets carried into indoor environments. Its ability to grow in such a wide range of niches is intriguing and needs further studies.
Since cryoEM of contracted AlgoCIS always showed empty inner tubes (Extended Data Fig. 7e and Supplementary Fig. 2c,d), we hypothesized that the Cgo1/Cgo2 proteins leak from the inner tube after contraction, similar to the cargo in MACs36. To corroborate this idea, we compared the preparations of wild-type AlgoCIS in extended vs contracted states by MS. Consistent with our hypothesis, Cgo1 was missing in the MS data of contracted AlgoCIS samples, while Cgo2 was detected with a significantly smaller number of unique peptides (Supplementary Table 2).
The phylogenetic trees of different contractile injection systems were examined using the putative sheath proteins on the basis of previous reports16. The amino acid sequences were first aligned by the MUSCLE online tool68,69 and further subjected to tree reconstruction in the MEGAX programme70. The maximum likelihood method and bootstrap values (1,000 resamples) were applied to assess the robustness of the tree.
The 1040ST is one of the earliest personal computers shipped with a base RAM configuration of 1 MB. With a list price of US$999 (equivalent to about $2,500 in 2021) in the US, BYTE hailed it as the first computer to break the $1000 per megabyte price barrier. Compute! noted that the 1040ST is the first computer with one megabyte of RAM to sell for less than $2,500.
In late 1989, Atari Corporation released the 520STE and 1040STE (also written STE), enhanced version of the ST with improvements to the multimedia hardware and operating system. It features an increased color palette of 4,096 colors from the ST's 512 (though the maximum displayable palette without programming tricks is still limited to 16 in the lowest 320 200 resolution, and even fewer in higher resolutions), Genlock support, and a blitter coprocessor (stylized as \"BLiTTER\") which can quickly move large blocks of data (particularly, graphics data) around in RAM. The STE is the first Atari with PCM audio; using a new chip, it added the ability to play back 8-bit (signed) samples at 6258 Hz, 12517 Hz, 25033 Hz, and even 50066 Hz, via direct memory access (DMA). The channels are arranged as either a mono track or a track of LRLRLRLR... bytes. RAM is now much more simply upgradable via SIMMs.
The final model of ST computer is the Falcon030. Like the TT, it is 68030-based, at 16 MHz, but with improved video modes and an on-board Motorola 56001 audio digital signal processor. Like the Atari STE, it supports sampling frequencies above 44.1 kHz; the sampling master clock is 98340 Hz (which can be divided by a number between 2 and 16 to get the actual sampling frequencies). It can play the STE sample frequencies (up to 50066 Hz) in 8 or 16 bit, mono or stereo, all by using the same DMA interface as the STE, with a few additions. It can both play back and record samples, with 8 mono channels and 4 stereo channels, allowing musicians to use it for recording to hard drive. Although the 68030 microprocessor can use 32-bit memory, the Falcon uses a 16-bit bus, which reduces performance and cost. In another cost-reduction measure, Atari shipped the Falcon in an inexpensive case much like that of the STF and STE. Aftermarket upgrade kits allow it to be put in a desktop or rack-mount case, with the keyboard separate.
Ten RT-qPCR assays were compared, a pre-existing in-house assay, three published assays and six newly designed assays, using serial RNA dilutions. We selected three assays, one published and two novel assays, with the lowest limit of detection (LOD) for further optimisation and validation. One of the novel assays, detecting NS2A, showed the best results, with LOD approximately 4 copies/ reaction, and no cross-reaction on testing closely related viruses in the JEV serocomplex, West Nile Virus and St. Louis Virus. The optimised assays were validated in consecutive patients with central nervous system infections admitted to hospitals in Laos, testing paired CSF and serum samples.
We aimed to perform a systematic review of the JEV RT-PCR assays available and to compare their performance. In addition, we developed new assays, in order to obtain the highest efficiency. This required extensive sequence analysis to design an in-silico optimal system followed by optimization of the experimental protocol. A set of selected assays were then assessed on patient samples.
The study was part of an ongoing study on the causes of CNS infections in Laos. Ethical clearance was granted by the Ethical Review Committee of the Faculty of Medical Sciences, National University of Laos, and the Oxford University Tropical Ethics Research Committee, Oxford, UK. Diagnostic testing was performed on anonymised and frozen serum and CSF samples previously collected. Venepuncture and LPs were performed if written consent was given by patients or their parents/guardian.
This systematic review confirmed that the detection of JEV RNA in suspected human cases by RT-PCR is uncommon. Forty-six studies were identified, involving a variety of RT-PCR techniques, however only a quarter had any published record of detection of JEV RNA in human samples. It was common for an article title to poorly identify the study as evaluating a JEV RT-PCR method, and while every effort was made to hand-search publications, it is possible that the search was not 100% comprehensive. Additionally, studies utilising the assays may not have cited them.
It is for these reasons that we focussed on identifying and validating the most effective RT-qPCR assay. Hydrolysis probe assays are the most extensively used methods for nucleic acid detection, both for clinical and research diagnostic purposes. From the results of the systematic review, 3 published systems incorporating hydrolysis probes were selected on the basis of best matching in silico. Assessment with JEV RNA dilutions of G1 and G3 showed the lowest LOD was obtained with the Pyke system: 2 logfold below both the Yang and Shirato assays with G1, and 1 logfold below both with G3 [27, 50]. It is recognised that the conditions employed were not identical to those published, and it would have been interesting to compare the validated assays with existing assays performed with published conditions. Validation experiments confirmed good RT-qPCR efficiency and LOD of 1.3 JEV RNA copies/μl or 39 copies/reaction. However, in a study of patient samples with paired serum a